Figure 4: SDS-PAGE analysis of cloned Hbγ-ECS1 expression. Partially purified recombinant Hbγ-ECS1, noninduced strain Rosetta/pET-sumo-Hbγ-ECS1, induced empty vector strain Rosetta/pET-sumo, wild E. coli Rosetta, cell supernatant of induced strain Rosetta/pET-sumo-Hbγ-ECS1, and protein ladder are present in lanes 1, 2, 3, 4, 5, and M, respectively.