Full-Length cDNA, Prokaryotic Expression, and Antimicrobial Activity of UuHb-F-I from Urechis unicinctus

Table 1

Primers used in this study.

Name	Sequences (5′-3′)	Purpose
Adaptor primer (Ap)	Containing the dT region designed by TaKaRa and adaptor primer part	3′-RACE cDNA
3′-RACE outer primer	TACCGTCGTTCCACTAGTGATTT	3′-RACE
3′-RACE inner primer	CGCGGATCCTCCACTAGTGATTTCACTATAGG	3′-RACE
Gene-specific primer (GSP1)	GGATATAGCGTTCTTTGACAAG	3′-RACE
Gene-specific primer (GSP2)	GCCCAGACTCTAACAGTTATCAGCTACTTGGAT	3′-RACE
SMARTer IIA oligo primers		5′-RACE cDNA
5′-RACE CDS primer A	(T)25VN	5′-RACE cDNA
10x universal primer	Long: CTAATACGACTCACTATAGGGC; AAGCAGTGGTATCAACGCAGAGT	5′-RACE
A Mix (UPM)	Short: CTAATACGACTCACTATAGGGC
5′-RACE outer primer	CATGGCTACATGCTGACAGCCTA	5′-RACE
5′-RACE inner primer	GCGGATCCACAGCCTACTGATGATCAGTCGATG	5′-RACE
Gene-specific primer (A1)	CATCATTACAGACCAGACAATACG	5′-RACE
Gene-specific primers (A2)	CGCTTCAAGAGTTGTCCGAAATGCTTCGTGGTG	5′-RACE
Primer P1	CAGGACGGAAGATATAGT	cDNA
Primer P2	GTCGTTGTGATGTAGCAG	cDNA
CDS-P1	GCGAGTCCATATG GGTCTTACTGGAGCTC	Recombinant expression
CDS-P2	TATACTCGAGCTTCATGGCGGCCACCAGG	Recombinant expression