Significant Associations of SOX9 Gene Polymorphism and Gene Expression with the Risk of Osteonecrosis of the Femoral Head in a Han Population in Northern China

Table 1

Primers and probes for the target SNP genotypes of SOX9 following LDR.

dbSNP			Product
rs12601701 [A/G]	Sense: TCATACAACTAAGTACAGACG	TA:TTTTTTGACACAGAAATAGGTCCACACTACA	61/A,
	Antisense: TGCATACAATAGCTTCGTATC	TG:TTTTTTTTTGACACAGAAATAGGTCCACACTACG; TR:-P-CGGACTTTTTTCTCCTAGGAAAGGATTTTT-FAM	64/G
rs1042667 [A/C]	Sense: CACTGGGAACAACCCGTCTAC	TA:TTTTTTTTTTGAGGAGGCCTCCCACGAAGGGCGAA	68/A,
	Antisense: TCCAAAGGGAATTCTGGTTGG	TC:TTTTTTTTTTTTTGAGGAGGCCTCCCACGAAGGGCGAC; TR:-P-GATGGCCGAGATGATCCTAAAAATAACCGAAGA-FAM	71/C

to amplify the gene fragment of the target dbSNP site.
to discriminate the target SNP genotype.
size indicates the size of the ligase reaction product from the different SNP genotypes.