Development of a Regenerative Peripheral Nerve Interface for Control of a Neuroprosthetic Limb

Figure 2

In situ image of Regenerative Peripheral Nerve Interface (RPNI), 4 months after implantation. In this example,  mm plates of myoblasts at culture day 14 were deposited on a one-layer thick sheet (2 cm long) acellular muscle. The common peroneal was transected, a 2 cm length was discarded, and the proximal residual end was tacked to the acellular muscle. The acellular muscle was rolled lengthwise to contain the myoblasts and maintain contact with the transected peroneal nerve. Evoked compound muscle action potential recording with stimulating electrode positioned on the peroneal nerve was 90 μV peak-to-peak.