Subtractive Cell-SELEX Selection of DNA Aptamers Binding Specifically and Selectively to Hepatocellular Carcinoma Cells with High Metastatic Potential

Figure 3

The specific binding affinity of these six selected aptamers to HCCLM9 cells. (a) The fluorescence imaging of six selected aptamers and initial ssDNA library control bound to HCCLM9 cells. Compared with the control group, all six selected aptamers were obviously bound to the membrane of target HCCLM9 cells; particularly, LY-1 and LY-13 had the highest binding capacity. (b) Binding curve of aptamers LY-1 and LY-46 with target HCCLM9 cells. Cells were incubated with increasing concentrations of FAM-labelled aptamers and the fluorescence intensity (MFI) was detected by flow cytometry. After subtracting the MFI of negative aptamer control (NK8), the MFI of FAM-aptamer bound target cells was used to calculate the value of each aptamer.

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