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BioMed Research International
Volume 2016, Article ID 5791510, 10 pages
Research Article

Activin A Stimulates Aromatase via the ALK4-Smad Pathway in Endometriosis

1State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China
2Center of Reproductive Medicine, 174th Hospital of PLA, Xiamen 361000, China

Received 21 May 2016; Accepted 17 August 2016

Academic Editor: Limei Qiu

Copyright © 2016 Juan Zheng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The protein and mRNA expression levels of P450arom from stromal cells in subjects with normal endometria and subjects with ovarian endometriosis were assessed by PCR and Western blot, respectively (Supplementary Figure). Figures A and C show representative data from three independent experiments. As shown in Figure B, the P450arom mRNA level was significantly increased in ectopic endometrial cells compared to normal and eutopic endometrial cells (p< 0.01). As illustrated in Figure D, the expression of P450arom protein in eutopic and ectopic tissues from patients diagnosed with endometriosis were significantly higher than in those with normal endometrial (p< 0.01). The difference in P450arom protein level between eutopic and ectopic tissues was apparent, but it was not statistically significant (p > 0.05).

  1. Supplementary Material