Establishment and Characterization of a Telomerase-Immortalized Sheep Trophoblast Cell Line

Figure 2

Retention of telomerase expression in hTERT-STCs. (a) Comparison of hTERT gene expression between hTERT-STCs and STCs by RT-PCR. M was the DL 500 DNA makers. Lane 1 was primary STCs; lane 2 was hTERT-STCs at passage 20; lane 3 was hTERT-STCs at passage 50; and lane 4 was HeLa cells (positive control). (b) Comparison of hTERT protein expression between hTERT-STCs and STCs by Western blot. Lanes 1–4 are the same as mentioned in (a). hTERT-STCs: human telomerase reverse transcriptase-sheep trophoblast cell line. RT-PCR: reverse transcriptase polymerase chain reaction.

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