Establishment and Characterization of a Telomerase-Immortalized Sheep Trophoblast Cell Line

Table 1

Primers and conditions used in RT-PCR gene expression.

Gene	Sequence (5′-3′)	Cycles	Product size (bp)	Anneal. temp. °C
hTERT		35	408	55
Sense	CGTACATGCGACAGTTCGTG
Antisense	AGTTCACCACTGTCTTCCGC
enJSRV-env		35	1836	60
Sense	ATGCCGAAGCGCCGCGCTGGATT
Antisense	TCACGGGTCGTCCCCCGCAGCTC
syncytin-Rum1		35	2014	60
Sense	GATGGAGCTGGGTAAGCGAC
Antisense	TTGTAGCCACGAGTTCCAGG
GAPDH		35	300	55
Sense	GTTTGTGATGGGCGTGAACC
Antisense	CCAGTGAGCTTCCCGTTGAG

RT-PCR: reverse transcriptase polymerase chain reaction; enJSRV-env: endogenous jaagsiekte sheep retrovirus envelope gene; and hTERT: human telomerase reverse transcriptase.