Comparative Proteomic Analysis of Light-Induced Mycelial Brown Film Formation in Lentinula edodes

Figure 2

Representative 2DE protein patterns from Lentinula edodes mycelium sample 313W that did not produce a brown film (a) and sample 313C (b) that produced a brown film. 313W indicates samples that did not form a brown film when cultivated in the dark, while 313C denotes samples that formed a brown film when cultivated under light/dark conditions. Proteins were loaded on a 24 cm IPG strip with a nonlinear immobilized pH gradient ranging from 3 to 10 for isoelectric focusing, followed by electrophoresis in a 12% SDS-PAGE gel and silver staining.

(a)

(b)