Comparative Proteomic Analysis of Light-Induced Mycelial Brown Film Formation in Lentinula edodes

Figure 4

Validation of proteomic data by RT-qPCR. Open and solid bars indicate fold changes obtained from proteomic data and by RT-qPCR, respectively. Error bars represent the mean ± SD of triplicate experiments. The full name of the gene evaluated by qPCR and their fold changes were 3-isopropylmalate dehydrogenase (idh, 1.841), NAD-aldehyde dehydrogenase (ndh, 1.054), nucleoside diphosphate kinase (ndk, 2.645), adenosine kinase (ake, −1.189), 20S proteasome subunit (pst, 1.998), proteasome component pts1 (pcp, 1.26), heat shock cognate 70 (hsc, 1.457), manganese superoxide dismutase (msd, 1.146), alcohol dehydrogenase (adh, −1.293), glyoxalase (gly, 1.247), and glutathione S-transferase-like protein (gtp, −1.549). The reference gene of qPCR was 18S rRNA.