Alendronate Can Improve Bone Alterations in Experimental Diabetes by Preventing Antiosteogenic, Antichondrogenic, and Proadipocytic Effects of AGEs on Bone Marrow Progenitor Cells

Figure 3

Effect of AGEs and/or alendronate on BMPC chondrocytic differentiation. BMPC were obtained from nondiabetic untreated animals and cultured for 21 days in a chondrogenic medium with 200 mg/mL of either control BSA or AGE-BSA, in the presence or absence of 10⁻⁸ M alendronate. Contrast-phase images of Alcian blue staining show intracellular glycosaminoglycans (GAGs) in the monolayer exposed to control BSA (a) or AGEs-BSA (b) (Obj. 40x). GAGs-associated stain was quantitated as a marker of chondrocytic differentiation (c). Results are expressed as the mean ± SEM. versus control BSA; versus AGEs-BSA.

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