Alendronate Can Improve Bone Alterations in Experimental Diabetes by Preventing Antiosteogenic, Antichondrogenic, and Proadipocytic Effects of AGEs on Bone Marrow Progenitor Cells

Figure 5

Effects of insulin-deficient Diabetes and oral treatment with alendronate on femoral microarchitecture. Diabetic and nondiabetic (control) animals were either left untreated (groups D and C) or treated for two weeks with 1 mg/kg/day of alendronate in their drinking water (groups DA and CA). Femora from animals of all groups were dissected, processed, and stained with hematoxylin-eosin or tartrate-resistant acid phosphatase histochemistry (TRAP). Proximal metaphysis was analyzed by quantitative histomorphometry to determine the following: (a) trabecular bone area, (b) trabecular osteocyte density, (c) relative osteoclastic TRAP activity in the secondary spongiosa, and (d) bone marrow adipocyte density. Results are expressed as the mean ± SEM. versus C; versus C; versus D; versus D.

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