Skeletal Muscle Remodelling as a Function of Disease Progression in Amyotrophic Lateral Sclerosis

Figure 4

Myogenesis in ALS muscle. mRNA transcripts of (a) MYF5, (b) PAX7, (c) MYOG, and (d) MYOD1 in ALS during disease progression compared to controls. (e and f) Representative immunohistochemical images of Pax7⁺ cells in ALS muscle. Arrows indicate Pax7⁺ cells associated with hypertrophied fibres and arrowheads indicate Pax7⁺ cells associated with atrophic fibres. (g–i) Double immunofluorescence stainings of MyoD (yellow), Pax7 (green), and DAPI (blue). (j–l) Double immunofluorescence stainings of MyoD (yellow), Ki67 (green), and DAPI (blue). (^∗) , , , and . In (a–d) for ALS and for controls and in (g–l) for ALS. Scale bar = 50 μm for all images.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)