Skeletal Muscle Remodelling as a Function of Disease Progression in Amyotrophic Lateral Sclerosis

Table 3

Analysis of activation status of satellite cells in ALS.

Subject	Pax7^+	Pax7^+/fibre	Pax7^+/MyoD^−	Pax7^+/MyoD^+	Ki67^+/MyoD^−
BL
1	13	0.071	12	2	1
2	32	0.066	30	2	2
3	26	0.074	23	3	0
4	11	0.092	11	0	1
5	22	0.068	22	0	0
Mean ± SEM	20.8 ± 3.9	0.074 ± 0.005	19.6 ± 3.6	1.4 ± 0.6	0.8 ± 0.37
12 wk.
1	10	0.057	9	1	0
2	10	0.067	9	1	0
3	21	0.088	21	0	1
4	23	0.099	23	0	0
5	25	0.091	25	0	0
Mean ± SEM	17.8 ± 3.25	0.080 ± 0.01	17.4 ± 3.5	0.4 ± 0.2	0.2 ± 0.2

Satellite cells/myoblast in BL and 12 wk. patient samples were stained for expression of Pax7, MyoD, and Ki67 using immunofluorescence and evaluated by counting. The number of identified cells in each section is noted.