Optimized Biotransformation of Icariin into Icariside II by β-Glucosidase from Trichoderma viride Using Central Composite Design Method

Figure 4

The results of monofactor experiments. (a) The reactions were carried out in sodium acetate-acetic acid buffer (pH 3.5, 4.0, 5.0, 6.0, 7.0, and 8.0) containing ICA (1 mg/mL) and enzyme (19.6 U/mL) at 40°C for 1 h. (b) The reactions were carried out in sodium acetate-acetic acid buffer (pH 5.0) containing ICA (1 mg/mL) and enzyme (19.6 U/mL) at 40°C for 0.5, 1, 1.5, 2, and 3 h. (c) The reactions were carried out in sodium acetate-acetic acid buffer (pH 5.0) containing ICA (1 mg/mL) and enzyme (19.6 U/mL) at 40, 45, 50, 55, and 60°C for 1 h. (d) The reactions were carried out in sodium acetate-acetic acid buffer (pH 5.0) containing ICA (1 mg/mL) and enzyme (1.96, 3.92, 9.8, 19.6, and 39.2 U/mL) at 40°C for 1 h. (e) The reactions were carried out in sodium acetate-acetic acid buffer (pH 5.0) containing ICA (0.1, 0.3, 0.5, 0.8, 1, and 2 mg/mL) enzyme (19.6 U/mL) at 40°C for 1 h.

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