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BioMed Research International
/
2016
/
Article
/
Tab 1
/
Research Article
Optimized Biotransformation of Icariin into Icariside II by
β
-Glucosidase from
Trichoderma viride
Using Central Composite Design Method
Table 1
Levels of variables used in the experimental design.
Independent variables
Levels
−1
0
1
pH (
)
3.32
4.0
5.0
6.0
6.68
Temperature (
) (°C)
23.18
30
40
50
56.86
Concentration of substrate (
) (mg mL
−1
)
0.30
0.30
0.65
1.00
1.24
: 1.682.