Heterologous, Expression, and Characterization of Thermostable Glucoamylase Derived from Aspergillus flavus NSH9 in Pichia pastoris

Figure 2

Starch-substrate assay plates showing halo/clear zone by the recombinant Mut⁺ Pichia colonies on soluble starch. Colonies 1, 2, and 4 were the recombinant GS115 and indicate the clear halo in plate; controls 1 and 2 did not produce any glucoamylase on the plate.