Heterologous, Expression, and Characterization of Thermostable Glucoamylase Derived from Aspergillus flavus NSH9 in Pichia pastoris

Figure 4

SDS-PAGE and native PAGE analysis of recombinant glucoamylase (GA). Lane 1 and Lane 2 are the 2nd day and 5th day of expression, respectively; Lane 3 indicates the purified recombinant glucoamylase; Lane 4 indicates control at 5th day; Lane 6 also indicates control for activity in native PAGE in 5th day; Lane 5 indicates activity for expressed GA in native PAGE (purified sample).