Heterologous, Expression, and Characterization of Thermostable Glucoamylase Derived from Aspergillus flavus NSH9 in Pichia pastoris

Table 1

Thermal stability of recombinant glucoamylase (GA) in different temperature by incubation of diluted enzyme with 0.1 M sodium acetate buffer pH 5.0 (1 : 1; 250 μL diluted enzyme in water : 250 μL 0.1 M sodium acetate buffer pH 5.0).

Time	Residual enzyme activity in different temperature
	60°C	70°C	80°C	90°C	100°C
0 min	100	100	100	100	100
5 min					83.28
10 min				95.89	77.73
15 min	98.25	96.98	91.53	88.16	58.67
20 min				51.49	49.63
25 min				36.64	33.38
30 min	80.82	70.89	32.8	25.02	20.15
45 min	50.3	39.87	25.32	14.57	12.02
60 min	24.15	11.12	11.0	8.7	0
75 min	11.94	5.82	3.0	0	0
90 min	8.45	0	0	0	0
120 min	0	0	0	0	0

Assay condition was set at optimum pH (5.0) and temperature (70°C). Each value represents the mean of three independent observations.