Association between TLR4 and PTEN Involved in LPS-TLR4 Signaling Response

Figure 1

PTEN promoted LPS-induced TNF-α secretion in RAW264.7 macrophages ( (a), (b)). (a) RAW264.7 cells were treated with or without PTEN inhibitor Bisperoxovanadium [bpV(Pic)] 100 nM for 1 hour, followed by the stimulation of 1 μg/mL LPS for 6 hours; concentrations of TNF-α in the supernatants were measured by ELISA. Macrophages treated with PTEN inhibitor produced significantly lower levels of TNF-α protein in comparison to macrophages without PTEN inhibitor (). (b) RAW264.7 cells were transfected with CMV (control empty vector), wild-type PTEN, G129E-PTEN (lipid phosphatase activity-disrupted mutant PTEN), and C124A-PTEN (lipid and protein phosphatases activity-disrupted mutant PTEN), cultured for 24 hours, and then stimulated with 1 μg/mL LPS for another 6 hours. The concentrations of TNF-α in the supernatants were measured by ELISA. Data are presented as the relative fold increase in LPS stimulated sample readout over nonstimulated sample readout and are expressed as the mean ± SD. Overexpression of wild-type PTEN enhanced LPS-induced TNF-α production in macrophages significantly (). However, overexpression of mutant PTEN with lipid phosphatase activity disruption and mutant PTEN with both lipid and protein phosphatases activity disruption did not show the difference with the empty plasmid control group (). versus bpV(Pic) (−) group; versus empty vector group.

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