Association between TLR4 and PTEN Involved in LPS-TLR4 Signaling Response

Figure 2

PTEN was activated and translocated to cell membrane after stimulation with LPS. At the same time, physical association between PTEN and TLR4 increased. (a) Phosphorylation levels of PTEN were reduced at 20, 30, and 45 minutes stimulated with LPS at 1 μg/mL. (b) The interaction between PTEN (red) and TLR4 (green), the PTEN in RAW264.7 cytoplasm redistributed towards cell membrane following the LPS stimulation. Scale bar = 15 μm. (c) Cell lysis was immunoprecipitated by TLR4 antibody, and western blot was probed by PTEN antibody. Goat IgG is worked as control. LPS 0 stands for without LPS treatment. The physical associations between PTEN and TLR4 were increased gradually and reached to their peak at 120 minutes after LPS stimulation. Translocation of PTEN in RAW264.7 cells was immunofluorescently stained at 0, 20, 60, and 120 minutes stimulated with LPS at 1 μg/mL by PTEN and TLR4 antibody. versus LPS 0 min group.

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