MP Resulting in Autophagic Cell Death of Microglia through Zinc Changes against Spinal Cord Injury

Figure 4

Effects of MP on microglia activation and secretion of inflammatory cytokines. (a) The expression of Iba-1 of microglia. After 12 hours of treatment, immunofluorescence staining was performed to assess expression of Iba-1 (Iba-1 green; DAPI blue); scale bar = 50 μm. (b) After 12 hours of incubation, the expression of protein iNOS in microglia was assessed in LPS group, LPS + MP group, and normal group by western blot. (c) Cytokine levels in culture medium. After 12 hours of treatment, the culture media were collected and expression of IL-6, IL-10, and TNF-α was detected by ELISA kit. Data were expressed as mean ± SD ( for each group). , versus normal group; versus LPS group.

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