18S Ribosomal RNA Evaluation as Preanalytical Quality Control for Animal DNA

Table 1

Chlamydiaceae negative samples, 18S rRNA qPCR.

Animal	Sample type	DNA (ng/µL)	OD 260/280	Mean Ct
Alpaca	Placenta	12.5	1.8	21.94
Goat	Liver and lung	188.3	1.9	15.48
Goat	Liver and lung	22.7	1.8	22.89
Guinea pig	Uterus swab	140.7	1.9	20.72
	Eye swab	307.4	1.9	18.41
Swine	Lung	304.3	1.8	14.44
	Spleen	274.3	1.9	14.90
Swine	Eye swab	1118.4	1.9	14.39
	Rectal swab	130.8	1.6	#
Turtle	Liver, lung, spleen	256.3	1.9	17.43

OD 260/280: absorbance at 260 nm/absorbance at 280 nm.
Mean Ct: mean cycle threshold, generated from duplicate wells.
Swabs were taken at necropsy.
Manual/kit extracted DNA; all other samples were automated/kit extracted.
FFPE sample; all other samples were taken from fresh tissues.
Undetected.