18S Ribosomal RNA Evaluation as Preanalytical Quality Control for Animal DNA

Table 2

Formalin-fixed paraffin-embedded liver samples, 18S rRNA qPCR.

Animal	DNA extraction method
	Manual/kit			Automated/kit
	DNA (ng/µL)	OD 260/280	Mean Ct	DNA (ng/µL)	OD 260/280	Mean Ct
Chinchilla	47.1	1.7	28.24	124.8	1.7	25.91
Chicken	49.1	1.9	27.83	78.2	1.8	25.32
Dog	195.7	1.9	22.44	139.9	1.7	20.38
Cat	219.9	2.0	21.42	128.7	1.7	18.84
Mouse	97.8	2.0	27.54	82.6	1.8	24.40
Guinea pig	63.1	1.9	23.74	125.0	1.8	21.93
Turtle	8.9	1.8	28.06	16.4	1.8	25.83

OD 260/280: absorbance at 260 nm/absorbance at 280 nm.
Mean Ct: mean cycle threshold, generated from duplicate wells.