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BioMed Research International
Volume 2016, Article ID 6106029, 15 pages
http://dx.doi.org/10.1155/2016/6106029
Research Article

Comparative Genomics of H. pylori and Non-Pylori Helicobacter Species to Identify New Regions Associated with Its Pathogenicity and Adaptability

1Center for Scientific Research, Youjiang Medical University for Nationalities, Baise, Guangxi 533000, China
2School of Medical Laboratory Sciences, Youjiang Medical University for Nationalities, Baise, Guangxi 533000, China
3School of Basic Medical Sciences, Youjiang Medical University for Nationalities, Baise, Guangxi 533000, China
4Department of Pathogenic Biology, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, Jiangsu 211166, China

Received 21 July 2016; Revised 17 September 2016; Accepted 11 October 2016

Academic Editor: Wen-Jun Li

Copyright © 2016 De-Min Cao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The genus Helicobacter is a group of Gram-negative, helical-shaped pathogens consisting of at least 36 bacterial species. Helicobacter pylori (H. pylori), infecting more than 50% of the human population, is considered as the major cause of gastritis, peptic ulcer, and gastric cancer. However, the genetic underpinnings of H. pylori that are responsible for its large scale epidemic and gastrointestinal environment adaption within human beings remain unclear. Core-pan genome analysis was performed among 75 representative H. pylori and 24 non-pylori Helicobacter genomes. There were 1173 conserved protein families of H. pylori and 673 of all 99 Helicobacter genus strains. We found 79 genome unique regions, a total of 202,359bp, shared by at least 80% of the H. pylori but lacked in non-pylori Helicobacter species. The operons, genes, and sRNAs within the H. pylori unique regions were considered as potential ones associated with its pathogenicity and adaptability, and the relativity among them has been partially confirmed by functional annotation analysis. However, functions of at least 54 genes and 10 sRNAs were still unclear. Our analysis of protein-protein interaction showed that 30 genes within them may have the cooperation relationship.