Different Levels of DNA Methylation Detected in Human Sperms after Morphological Selection Using High Magnification Microscopy

Figure 1

Spermatozoa classification and 5-methylcytosine immune labeling in S0 and S6 spermatozoa (a): “high-scoring” spermatozoon Score 6 at high magnification (6100x). (b): “low-scoring” spermatozoon Score 0 at high magnification (6100x). Cassuto Barak Classification for motile sperm cells. Fertil Steril 2009. (c): 5-methylcytosine detection in S6 spermatozoa (100x). Merge Dapi (Blue) and FITC (Green). Spermatozoa showing green labeling display detectable level of DNA methylation. (d): 5-methylcytosine detection in S6 spermatozoa (100x). Merge Dapi (Blue) and FITC (Green). Spermatozoa showing green labeling display detectable level of DNA methylation.

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