Multiple Genetic Associations with Irish Wolfhound Dilated Cardiomyopathy

Table 1

PCR primer information.

SNP	Primer pair	Forward primer sequence	Reverse primer sequence	PCR fragment length
Chr1: rs21953123 C/T	Initial primers	TCCTCTCCAAACATTAGAAAAAGACC	ACTACATATAGTCAGCTTTACAGATTG	311 bp
	Additional primers	TATCGTACTTTATAATGAAAGATTGAATG	AGAATTTAATATCTGAAAATATATCAACAC	417 bp
Chr10: rs22078677 A/C	Initial primers	CCTGGTGTCAGAAACACAAGgCAC	TCAAGTAGCTTAAACTCTCAGGGCC	374 bp
	Initial forward primer, additional reverse primer		TGTGTATTCATCAGCTCTTCCTAGG	161 bp
Chr15: rs22422063 C/A	Initial primers	AATCATTTGACAGTTAGGTTAACTTCC	CACAATTTGCACATTACACATTATTAAC	375 bp
	initial forward primer, additional reverse primer	GCTGGTAAACATTCTTAAATTCAGCG	ATTTGAGTTTTCCCTCACATTATTGAC	443 bp
Chr21: rs22923291 G/A	Initial primers	GAAACTCCAGTCATGTTTAACTATTTG	GAAGAGCAGTAATGACTAATACgCAG	349 bp
	additional forward primer, initial reverse primer	TGTTCATCTCTCACAGGACTTGAG		410 bp
Chr37: rs24025150 G/A	Initial primers	GGAAACTGGTTTCCAATGAAGAcC	ACGTAACAAGTTTGAAGTGTTGCTG	374 bp
	initial forward primer, additional reverse primer		CCTGCCACCTGTGTTACTTTACGC	133 bp

DCM associated SNP locations in genome (from Philipp et al. [23]), PCR primer sequence information, if the primer pair is the initial or new primers, and resulting PCR product sizes. Primer sequence in lower case indicates a base change from the reference sequence to create a restriction digest site.