Multiple Genetic Associations with Irish Wolfhound Dilated Cardiomyopathy

Table 3

Restriction digest information.

SNP	Enzyme	Units of enzyme per reaction	Restriction site	Cuts allele	Primer pair	Digest fragment sizes
Chr1: rs21953123 C/T	HpyCH4II (NEB, USA)	3	ACTGT	C	Original primers	228 & 83
					New primers	260 & 157
Chr10: rs22078677 A/C	BanI (NEB, USA)	6	GgCACC	C	Original primers	19 & 355
					Original forward primer, new reverse primer	19 & 142
Chr15: rs22422063 C/A	NciI (NEB, USA)	6	CCCGG	C	Original primers	196 & 179
					Original forward primer, new reverse primer	227 & 216
Chr21: rs22923291 G/A	Fnu4HI (NEB, USA)	1	GCTGc	G	Original primers	310 & 39
					New forward primer, original reverse primer	384 & 39
Chr37: rs24025150 G/A	HpaII (NEB, USA)	3	cCGG	G	Original primers	28 & 346
					Original forward primer, new reverse primer	28 & 105

Enzymes, restriction sites, primer pairs, and digest fragment sizes for each SNP.