Research Article
Purification and Characterization of a Thermostable β-Mannanase from Bacillus subtilis BE-91: Potential Application in Inflammatory Diseases
Table 2
Purification of β-mannanase by ultrafiltration and gel chromatography.
| | Purification step | Total activity (IU) | Total protein (mg) | Specific activity (IU/mg) | Recovery (%) | Purification multiple (fold) |
| | Fermentation liquor | 429,650.8 | 176.7 | 2,431.4 | 100 | 1 | | Ultrafiltration | 328,317.4 | 8.6 | 38,070.2 | 76.4 | 15.6 | | Gel chromatography | 283,500.2 | 3.6 | 79,859.2 | 66.0 | 32.9 |
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