Efficiency of Human Epiphyseal Chondrocytes with Differential Replication Numbers for Cellular Therapy Products

Table 2

Primer pairs and experimental conditions for RT-PCR.

Gene product	Forward and reverse primers (5′-3′)	Expected product size (bp)
Collagen I	CCCAAGGACAAGAGGCAT	159
	GCAGTGGTAGGTGATGTTCTG
Collagen II	AGATGGCTGGAGGATTTGAT	301
	CTTGGATAACCTCTGTGACTT
Collagen III	GGGTCCTACTGGTCCTATTGG	229
	TCGAAGCCGAATTCCTGGTCT
Collagen X	AGCCAGGGTTGCCAGGACCA	387
	TTTTCCCACTCCAGGAGGGC
β2-microglobulin	CTCGCGCTACTCTCTCTTTCTGG	335
	GCTTACATGTCTCGATCCCACTTAA