Cogels of Hyaluronic Acid and Acellular Matrix for Cultivation of Adipose-Derived Stem Cells: Potential Application for Vocal Fold Tissue Engineering

Figure 2

Cell morphology of hADSCs in 3D hydrogel was determined in the cell culture. (a) hADSCs were shown to be circular at 0 h in HA-DCM composite hydrogel; (b) hADSCs developed into spindle cells with fibroblasts-like shape at 24 h; (c–e) cell shape of hADSCs that were labeled with GFP in HA, HA-Col, and HA-DCM hydrogel was observed at the 7th day after cell seeding. The arrows indicated that hADSCs in HA, HA-Col, and HA-DCM hydrogel were shown to be ellipsoid at the 7th day. (f) Quantification of cell shape of hADSCs indicated that the major/minor length of hADSCs in HA-DCM hydrogel was shown to be larger than one in HA-Col hydrogel, and the major/minor length of hADSCs in HA hydrogel was shown to be the smallest in the three groups. indicated .

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