Development of Monoclonal Antibodies against HIV-1 p24 Protein and Its Application in Colloidal Gold Immunochromatographic Assay for HIV-1 Detection

Figure 1

Preparation and identification of recombinant p24. (a) Expression and purification of recombinant p24. 1: whole cell lysate without IPTG induction, 2: whole cell lysate with IPTG induction, and 3: flow through solution; lanes 4 to 7 are four different solutions eluted by 50 mM, 150 mM, 300 mM, and 500 mM imidazole buffer, respectively. (b) Indirect ELISA analysis of the recombinant p24. A: antip24 antibody as the primary antibody, B: blank without primary antibody, C: blank without coating antigen, and D: blank without primary antibody and coating antigen.

(a)

(b)