Research Article
Development of Monoclonal Antibodies against HIV-1 p24 Protein and Its Application in Colloidal Gold Immunochromatographic Assay for HIV-1 Detection
Table 1
The detection results of National Reference for HIV-1 p24 antigen by GICA strips.
| | Sample | Derivation of references | Genotype | Detection level |
| | P1 | P24 positive Sample 34# | AE | Faint | | P2 | P24 positive Sample 106# | B | Faint | | P3 | P24 positive Sample 116# | B | Medium | | P4 | P24 positive Sample 119# | B | Weak | | P5 | 500 fold-diluted laboratory-grown virus sample A | Not given | Medium | | P6 | 100 fold-diluted laboratory-grown virus sample A | Not given | Weak | | P7 | 1000 fold-diluted laboratory-grown virus sample L | B | Faint | | P8 | 10000 fold-diluted laboratory-grown virus sample L | B | Faint | | P9 | 50 fold-diluted HIV-1 window period Sample P | AE | Faint | | P10 | WHO Standard p24 25 U/mL | B | Medium | | L1 | WHO Standard p24 20 U/mL | B | Medium | | L2 | WHO Standard p24 10 U/mL | B | Weak | | L3 | WHO Standard p24 5 U/mL | B | Faint | | L4 | WHO Standard p24 2.5 U/mL | B | Negative | | L10 | Buffer for L Series | B | Negative |
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Detection level: Band intensity was read independently by two individuals.
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