BioMed Research International / 2016 / Article / Tab 1

Research Article

Development of Monoclonal Antibodies against HIV-1 p24 Protein and Its Application in Colloidal Gold Immunochromatographic Assay for HIV-1 Detection

Table 1

The detection results of National Reference for HIV-1 p24 antigen by GICA strips.

SampleDerivation of referencesGenotypeDetection level

P1P24 positive Sample 34#AEFaint
P2P24 positive Sample 106#BFaint
P3P24 positive Sample 116#BMedium
P4P24 positive Sample 119#BWeak
P5500 fold-diluted laboratory-grown virus sample ANot givenMedium
P6100 fold-diluted laboratory-grown virus sample ANot givenWeak
P71000 fold-diluted laboratory-grown virus sample LBFaint
P810000 fold-diluted laboratory-grown virus sample LBFaint
P950 fold-diluted HIV-1 window period Sample PAEFaint
P10WHO Standard p24 25 U/mLBMedium
L1WHO Standard p24 20 U/mLBMedium
L2WHO Standard p24 10 U/mLBWeak
L3WHO Standard p24 5 U/mLBFaint
L4WHO Standard p24 2.5 U/mLBNegative
L10Buffer for L SeriesBNegative

Detection level: Band intensity was read independently by two individuals.

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