Near-Infrared Fluorescence Imaging of Carbonic Anhydrase IX in Athymic Mice Bearing HT-29 Tumor Xenografts

Figure 2

In vitro binding characteristics of Mab-Cy5.5. RCC4 cells were incubated with (a) 10 nM Mab-Cy5.5 and 100 nM free Mab under hypoxia; (b) 10 nM Mab-Cy5.5 and 100 nM free Mab under normoxia; (c) 100 nM free Cy5.5 under hypoxia; (d) 100 nM free Cy5.5 under normoxia.

(a)

(b)

(c)

(d)