Interaction Effects between Organochlorine Pesticides and Isoflavones In Vitro and In Vivo

Figure 3

Interaction of isoflavones and γ-HCH on uterine morphology and expression of ERα. (a) showed interaction of isoflavones and γ-HCH on uterine morphology. Compared with control group (group 9), high columnar endometrial glandular epithelial cells were found in group 3, while low columnar endometrial glandular epithelial cells were shown in group 7. Furthermore, although high columnar endometrial glandular epithelial cells were found in group 1, the degree of hyperplasia was lower than isoflavones treatment group and higher than γ-HCH treatment group. (b) showed interaction of isoflavones and γ-HCH on expression of ERα. Compared to group 9, ERα expression was higher in group 3 (), while being lower in group 7 (). Moreover, ERα expression in group 1 was lower than group 3 and higher than group 7. (c) showed positive expression of ERα in immunohistochemistry. value < 0.05 was considered significant compared with control group (group 9). Group 1 was treated with 900 mg/kg diet isoflavones and 8 mg/kg bw γ-HCH; group 3 was treated with 900 mg/kg diet isoflavones alone; group 7 was treated with 8 mg/kg bw γ-HCH alone; group 9 was treated with 0 mg/kg diet isoflavones and 0 mg/kg bw γ-HCH.

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