Molecular Diagnosis and Identification of Leishmania Species in Jordan from Saved Dry Samples

Figure 1

Representative pictures showing agarose gel electrophoresis (2%) of random PCR products (300–350 bp) which were extracted from the 30 positive Leishmania samples. Lane M: 100 bp DNA ladder. Lanes 1–12: PCR products randomly selected from 30 clinical samples (a) and (b) showing the digestion of amplified ITS1 regions for different Leishmania species with the restriction endonuclease Hae IIII. Lane M: 100 bp DNA ladder. Lane 1: negative control. Lane 2: healthy individual control. Lane 3: L. major positive control showing two bands (203 bp and 132 bp). Lane 4: L. tropica positive control showing three bands (185 bp, 57 bp, and 24 bp). Lanes 5, 6, 7, 8, 9, 10, 11, 13, 14, and 15: random samples for L. major detected in clinical samples. Lane 12: L. tropica detected in clinical samples.

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