Skin-Derived Precursors against UVB-Induced Apoptosis via Bcl-2 and Nrf2 Upregulation

Figure 3

Differentiation of SKPs into osteocytes and adipocytes. (a) After being cultured for 3 weeks in osteogenesis induction medium, SKPs differentiated into bone-like cells with central aggregation and fusion, as well as multiple high-density calcium nodules which were stained yellowish red by Alizarin Red Solution (arrows indicated calcium nodules positively stained by Alizarin Red Solution). (b) After being cultured for 3 weeks in adipogenesis induction medium, SKPs differentiated into round or vacuolated cells with lipid droplets, which were in part stained bright red by Oil Red Solution (arrows indicated vacuolated cells positively stained by Oil Red Solution). (c) and (d) Both as control, after being cultured in medium without inducer, SKPs were not stained by either Alizarin Red Solution or Oil Red Solution.

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