Usefulness of Multiplex Real-Time PCR for Simultaneous Pathogen Detection and Resistance Profiling of Staphylococcal Bacteremia

Table 1

PCR primers and TaqMan probes for mecA, femA specific for S. aureus (femA-SA), femA specific for S. epidermidis (femA-SE), and universal 16S rRNA and PCR primers for staphylococcal 16S rRNA.

Target genes	Sequence
mecA	5′-CATTGATCGCAACGTTCAATTT-3′
	5′-TGGTCTTTCTGCATTCCTGGA-3′
	5′-FAM-TGGAAGTTAGATTGGGATCATAGCGTCAT-TAMRA-3′
femA-SA	5′-TGCCTTTACAGATAGCATGCCA-3′
	5′-AGTAAGTAAGCAAGCTGCAATGACC-3′
	5′-JOE-TCATTTCACGCAAACTGTTGGCCACTATG-BHQ1-3′
femA-SE	5′-CAACTCGATGCAAATCAGCAA-3′
	5′-GAACCGCATAGCTCCCTGC-3′
	5′-JOE-TACTACGCTGGTGGAACTTCAAATCGTTATCG-BHQ1-3′
Universal 16S rRNA	5′-TCCTACGGGAGGCAGCAGT-3′
	5′-GGACTACCAGGGTATCTAATCCTGTT-3′
	5′-FAM-CGTATTACCGCGGCTGCTGGCAC-TAMRA-3′
Staphylococcal 16S rRNA	5′-GCAAGCGTTATCCGGATTT-3′
	5′-CTTAATGATGGCAACTAAGC-3′