C3a Increases VEGF and Decreases PEDF mRNA Levels in Human Retinal Pigment Epithelial Cells

Figure 3

Effect of siRNA targeting C3 on the level of VEGF (a) and PEDF (b) mRNA in cultured ARPE-19 cells. Total RNA was extracted and mRNA evaluated by RT-PCR analysis. The ratio of the abundance of each mRNA to that of GAPDH was evaluated by densitometric analysis. Data are mean ± SD (). (a) The VEGF mRNA was significantly lower in the ARPE-19 cells transfected with 0.1 pmol/μL siRNA targeting C3 for 48 hours compared to control siRNA; (b) the PEDF mRNA was significantly higher in the ARPE-19 cells after transfection of 0.1 pmol/μL siRNA targeting C3 for 48 hours compared to that of transfection with control siRNA. versus control siRNA transfected cells.

(a)

(b)