ZFP91: A Noncanonical NF-κB Signaling Pathway Regulator with Oncogenic Properties Is Overexpressed in Prostate Cancer

Figure 3

Transfection of LNCaP cell line with ZFP91 siRNA. Top. cells transfected with siGLO Green Transfection Indicator showing good transfection efficiency. Representative cell images under bright field (a), fluorescence field with a FITC filter (b), and superimposed images (c). (d) QPCR analysis of ZFP91 gene RNA interference. Cells transfected with ZFP91 siRNA pool (siZFP), nontargeting siRNA control pool (siNEG), and untreated (Un). ZFP91 mRNA levels at different time points were examined. Bars represent mean ZFP91 expression ± SE; median value in untreated group was assigned a value of 100. Each experiment was performed in triplicate.

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