Identification and Evolutionary Analysis of Potential Candidate Genes in a Human Eating Disorder

Figure 3

How nonsynonymous mutations are tolerated within VGF, CALR, and HTRA1 proteins. Predictions of functional consequences are determined by SIFT and PolyPhen-2. Darker regions are predicted to be “tolerated” or “benign” by both SIFT and PolyPhen-2, respectively. Bars with a lighter shade depict those mutations which are predicted to be “deleterious” and “probably damaging,” with the exclusion of “probably damaging” (lower confidence score) assessments from PolyPhen-2.