The Antitumor Effect of Gekko Sulfated Glycopeptide by Inhibiting bFGF-Induced Lymphangiogenesis

Figure 3

GSPP inhibits bFGF-induced lymphangiogenesis in vitro. In vitro tube formation assay. hLECs (1.5 × 10⁴ cells/well) were seeded in Matrigel-coated 96-well plates and treated with different concentration of GSPP (0, 10, and 100 μg/mL) and/or bFGF (10 ng/mL) for 4 h and the tube-like structure formation was observed. (a) Representative images of tube formation (×40). (b) Quantification of inhibitory ratios of tube branches. (A) Control, (B) bFGF 10 ng/mL, (C) GSPP 10 µg/mL, (D) GSPP 100 µg/mL, (E) GSPP 10 µg/mL + bFGF 10 ng/mL, and (F) GSPP 100 µg/mL + bFGF 10 ng/mL. Data were presented as mean ± SD of three independent experiments; versus control group and versus bFGF-single use group.

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