The Antitumor Effect of Gekko Sulfated Glycopeptide by Inhibiting bFGF-Induced Lymphangiogenesis

Figure 4

GSPP inhibited colon carcinoma HT-29 xenograft growth and lymphangiogenesis in vivo. Male nu/nu nude mice were inoculated subcutaneously with colon carcinoma HT-29 cells. Three days after inoculation, mice were treated with GSPP (20 or 200 mg/kg) or PBS every day for 21 days via intraperitoneal injection. The lengths and widths of tumors were measured individually every 3 days. At the end of the experiment, the implanted tumors were sectioned. (a) Effect of GSPP on tumor volume. Left, image of excised tumors. Right, tumor growth curves. (b) Effect of GSPP on tumor weight. (c) Effect of GSPP on mouse weight. Mice were weighed at the end of the experiment. (d) Tumor lymphatic microvessel density. The implanted tumors were sectioned and stained against LYNE-1 antibody. Tumor lymphatic vessels are shown as LYNE-1 positive (yellow color). Data were presented as mean ± SD of three independent experiments; versus control group.

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