Magnesium Attenuates Phosphate-Induced Deregulation of a MicroRNA Signature and Prevents Modulation of Smad1 and Osterix during the Course of Vascular Calcification

Figure 3

miR-30b, miR-133a, and miR-143 expression is deregulated by Pi and restored by Mg²⁺. Pi-induced HAVSMC were assessed at the time points 4 h, 24 h, day 3, day 7, and day 10. miR-30b (a), miR-133a (b), and miR-143 (c) were quantified using TaqMan microRNA assays (Applied Biosystem) on total RNA by q-PCR analysis and compared to control cells treated with basal medium. The control condition was set to 1 (dashed line). The cumulated relative quantity (RQ) of the three different donors of HAVSMC is represented as a ratio of the control condition and expressed as mean ± SEM for each condition (). U6 small nuclear RNA was used as an internal reference gene. Statistics were realised with the Mann-Whitney test. versus Ct, versus Ct, and versus Pi.

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