Partial Portal Vein Arterialization Attenuates Acute Bile Duct Injury Induced by Hepatic Dearterialization in a Rat Model

Figure 5

PPVA reduced BDI-induced ductular reaction and fibrosis. Representative microscopic images of Sirius Red staining (a) and alpha-smooth muscle actin (α-SMA) immunostaining (b) are shown 14 days after surgery (magnification, 100x). (c) The magnitude of the ductular reaction was reduced by PPVA (bile ducts in 10 portal tract-centered areas were counted in high magnification in each group). (d) Procollagen I mRNA expression was evaluated using qRT-PCR. (e) Collagen content was evaluated by measurement of liver hydroxyproline. (f) Quantification of α-SMA immunostaining area was accessed by Image-Pro Plus software. (g) PV pressure was measured in all groups to examine whether PPVA induced PV hypertension. PCR results are expressed as the mean ± SEM of at least triplicate measurements. versus the Sham group; versus the PPVA + BDI group. Differences were assessed by two-way ANOVA and comparisons between groups were performed by Bonferroni posttests at each time point.

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