Partial Portal Vein Arterialization Attenuates Acute Bile Duct Injury Induced by Hepatic Dearterialization in a Rat Model

Figure 6

PPVA improved bile duct hypoxia after hepatic arterial deprivation. (a) Representative HIF-1α immunostaining images are shown. Karyotypic HIF-1α expression (arrow) in the BDI group and cytoplasmic HIF-1α expression (arrowheads) in the PPVA + BDI group were detected in the bile duct cells 1 day after the surgery (magnification, 200x). (b) Hepatic HIF-1α and VEGF mRNA expression was analyzed using qRT-PCR. (c) Total protein extracts were subjected to western blotting. Representative gels showed that BDI increased HIF-1α protein expression, which was reduced by PPVA. PCR results are expressed as the mean ± SEM of at least triplicate measurements. Western blotting data are the mean ± SEM of three independent experiments and are normalized to GAPDH. versus the Sham group; versus the PPVA + BDI group. Differences were assessed by two-way ANOVA and comparisons between groups were performed by Bonferroni posttests at each time point.

(a)

(b)

(c)