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BioMed Research International
Volume 2016, Article ID 7432131, 8 pages
http://dx.doi.org/10.1155/2016/7432131
Review Article

In Vivo Laser Scanning Confocal Microscopy of Human Meibomian Glands in Aging and Ocular Surface Diseases

1Ophthalmology Clinic, Department of Medicine and Aging Science, “G. d’Annunzio” University of Chieti-Pescara, 66100 Chieti, Italy
2Ophthalmology Unit, Department of Neurological, Neuropsychological, Morphological and Movement Sciences, University of Verona, 37126 Verona, Italy
3Ophthalmic Clinic, Department of Life, Health and Environmental Sciences, University of L’Aquila, 67100 L’Aquila, Italy

Received 13 November 2015; Revised 8 February 2016; Accepted 17 February 2016

Academic Editor: Dipika V. Patel

Copyright © 2016 Vincenzo Fasanella et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. E. Knop, N. Knop, T. Millar, H. Obata, and D. A. Sullivan, “The international workshop on meibomian gland dysfunction: report of the subcommittee on anatomy, physiology, and pathophysiology of the meibomian gland,” Investigative Ophthalmology and Visual Science, vol. 52, no. 4, pp. 1938–1978, 2011. View at Publisher · View at Google Scholar · View at Scopus
  2. S. Mishima and D. M. Maurice, “The oily layer of the tear film and evaporation from the corneal surface,” Experimental Eye Research, vol. 1, pp. 39–45, 1961. View at Publisher · View at Google Scholar · View at Scopus
  3. J. M. Tiffany, “The lipid secretion of the meibomian glands,” Advances in Lipid Research, vol. 22, pp. 1–62, 1987. View at Google Scholar · View at Scopus
  4. J. V. Jester, L. Rife, D. Nii, J. K. Luttrull, L. Wilson, and R. E. Smith, “In vivo biomicroscopy and photography of meibomian glands in a rabbit model of meibomian gland dysfunction,” Investigative Ophthalmology and Visual Science, vol. 22, no. 5, pp. 660–677, 1982. View at Google Scholar · View at Scopus
  5. R. Mastropasqua, V. Fasanella, E. Pedrotti et al., “Trans-conjunctival aqueous humor outflow in glaucomatous patients treated with prostaglandin analogues: an in vivo confocal microscopy study,” Graefe's Archive for Clinical and Experimental Ophthalmology, vol. 252, no. 9, pp. 1469–1479, 2014. View at Publisher · View at Google Scholar · View at Scopus
  6. L. Agnifili, P. Carpineto, V. Fasanella et al., “Conjunctival findings in hyperbaric and low-tension glaucoma: an in vivo confocal microscopy study,” Acta Ophthalmologica, vol. 90, no. 2, pp. e132–e137, 2012. View at Publisher · View at Google Scholar · View at Scopus
  7. L. Mastropasqua, L. Agnifili, V. Fasanella et al., “Conjunctival goblet cells density and preservative-free tafluprost therapy for glaucoma: an in vivo confocal microscopy and impression cytology study,” Acta Ophthalmologica, vol. 91, no. 5, pp. e397–e405, 2013. View at Publisher · View at Google Scholar · View at Scopus
  8. L. Mastropasqua, M. Nubile, M. Lanzini et al., “Morphological modification of the cornea after standard and transepithelial corneal cross-linking as imaged by anterior segment optical coherence tomography and laser scanning in vivo confocal microscopy,” Cornea, vol. 32, no. 6, pp. 855–861, 2013. View at Publisher · View at Google Scholar · View at Scopus
  9. L. Agnifili, V. Fasanella, S. D'Aguanno et al., “Shotgun proteomics reveals specific modulated protein patterns in tears of patients with primary open angle glaucoma naïve to therapy,” Molecular BioSystems, vol. 9, no. 6, pp. 1108–1116, 2013. View at Publisher · View at Google Scholar · View at Scopus
  10. R. Mastropasqua, L. Agnifili, V. Fasanella et al., “Corneoscleral limbus in glaucoma patients: in vivo confocal microscopy and immunocytological study,” Investigative Opthalmology & Visual Science, vol. 56, no. 3, pp. 2050–2058, 2015. View at Publisher · View at Google Scholar
  11. P. Carpineto, L. Agnifili, M. Nubile et al., “Conjunctival and corneal findings in bleb-associated endophthalmitis: an in vivo confocal microscopy study,” Acta Ophthalmologica, vol. 89, no. 4, pp. 388–395, 2011. View at Publisher · View at Google Scholar · View at Scopus
  12. L. Mastropasqua, L. Agnifili, R. Mastropasqua et al., “In vivo laser scanning confocal microscopy of the ocular surface in glaucoma,” Microscopy and Microanalysis, vol. 20, no. 3, pp. 879–894, 2014. View at Publisher · View at Google Scholar · View at Scopus
  13. E. Villani, C. Baudouin, N. Efron et al., “In vivo confocal microscopy of the ocular surface: from bench to bedside,” Current Eye Research, vol. 39, no. 3, pp. 213–231, 2014. View at Publisher · View at Google Scholar · View at Scopus
  14. N. Efron, M. Al-Dossari, and N. Pritchard, “In vivo confocal microscopy of the palpebral conjunctiva and tarsal plate,” Optometry and Vision Science, vol. 86, no. 11, pp. E1303–E1308, 2009. View at Publisher · View at Google Scholar · View at Scopus
  15. A. Wei, J. Hong, X. Sun, and J. Xu, “Evaluation of age-related changes in human palpebral conjunctiva and meibomian glands by in vivo confocal microscopy,” Cornea, vol. 30, no. 9, pp. 1007–1012, 2011. View at Publisher · View at Google Scholar · View at Scopus
  16. E. Villani, V. Canton, F. Magnani, F. Viola, P. Nucci, and R. Ratiglia, “The aging meibomian gland: an in vivo confocal study,” Investigative Ophthalmology and Visual Science, vol. 54, no. 7, pp. 4735–4740, 2013. View at Publisher · View at Google Scholar · View at Scopus
  17. D. A. Sullivan, B. D. Sullivan, M. D. Ullman et al., “Androgen influence on the meibomian gland,” Investigative Ophthalmology and Visual Science, vol. 41, no. 12, pp. 3732–3742, 2000. View at Google Scholar · View at Scopus
  18. B. D. Sullivan, J. E. Evans, J. M. Cermak, K. L. Krenzer, M. R. Dana, and D. A. Sullivan, “Complete androgen insensitivity syndrome: effect on human meibomian gland secretions,” Archives of Ophthalmology, vol. 120, no. 12, pp. 1689–1699, 2002. View at Publisher · View at Google Scholar · View at Scopus
  19. C. Auw-Haedrich and N. Feltgen, “Estrogen receptor expression in meibomian glands and its correlation with age and dry-eye parameters,” Graefe's Archive for Clinical and Experimental Ophthalmology, vol. 241, no. 9, pp. 705–709, 2003. View at Publisher · View at Google Scholar · View at Scopus
  20. M. M. Hom, J. R. Martinson, L. L. Knapp, and J. R. Paugh, “Prevalence of meibomian gland dysfunction,” Optometry and Vision Science, vol. 67, no. 9, pp. 710–712, 1990. View at Publisher · View at Google Scholar · View at Scopus
  21. A. Bodineau, M. Folliguet, and S. Séguier, “Tissular senescence and modifications of oral ecosystem in the elderly: risk factors for mucosal pathologies,” Current Aging Science, vol. 2, no. 2, pp. 109–120, 2009. View at Publisher · View at Google Scholar · View at Scopus
  22. E. M. Rocha, M. Alves, J. D. Rios, and D. A. Dartt, “The aging lacrimal gland: changes in structure and function,” Ocular Surface, vol. 6, no. 4, pp. 162–174, 2008. View at Publisher · View at Google Scholar · View at Scopus
  23. L. Agnifili, R. Mastropasqua, V. Fasanella et al., “In vivo confocal microscopy of conjunctiva-associated lymphoid tissue in healthy humans,” Investigative Ophthalmology and Visual Science, vol. 55, no. 8, pp. 5254–5262, 2014. View at Publisher · View at Google Scholar · View at Scopus
  24. E. Villani, F. Magnani, F. Viola et al., “In vivo confocal evaluation of the ocular surface morpho-functional unit in dry eye,” Optometry and Vision Science, vol. 90, no. 6, pp. 576–586, 2013. View at Publisher · View at Google Scholar · View at Scopus
  25. E. Villani, F. Mantelli, and P. Nucci, “In-vivo confocal microscopy of the ocular surface: ocular allergy and dry eye,” Current Opinion in Allergy and Clinical Immunology, vol. 13, no. 5, pp. 569–576, 2013. View at Publisher · View at Google Scholar · View at Scopus
  26. A. Kobayashi, T. Yoshita, and K. Sugiyama, “In vivo findings of the bulbar/palpebral conjunctiva and presumed meibomian glands by laser scanning confocal microscopy,” Cornea, vol. 24, no. 8, pp. 985–988, 2005. View at Publisher · View at Google Scholar · View at Scopus
  27. E. M. Messmer, M. J. Mackert, D. M. Zapp, and A. Kampik, “In vivo confocal microscopy of normal conjunctiva and conjunctivitis,” Cornea, vol. 25, no. 7, pp. 781–788, 2006. View at Publisher · View at Google Scholar · View at Scopus
  28. E. M. Messmer, E. Torres Suárez, M. I. Mackert, D. M. Zapp, and A. Kampik, “In vivo confocal microscopy in blepharitis,” Klinische Monatsblatter fur Augenheilkunde, vol. 222, no. 11, pp. 894–900, 2005. View at Publisher · View at Google Scholar · View at Scopus
  29. Y. Matsumoto, E. A. Sato, O. M. A. Ibrahim, M. Dogru, and K. Tsubota, “The application of in vivo laser confocal microscopy to the diagnosis and evaluation of meibomian gland dysfunction,” Molecular Vision, vol. 14, pp. 1263–1271, 2008. View at Google Scholar · View at Scopus
  30. O. M. A. Ibrahim, Y. Matsumoto, M. Dogru et al., “The efficacy, sensitivity, and specificity of in vivo laser confocal microscopy in the diagnosis of meibomian gland dysfunction,” Ophthalmology, vol. 117, no. 4, pp. 665–672, 2010. View at Publisher · View at Google Scholar · View at Scopus
  31. O. M. A. Ibrahim, Y. Matsumoto, M. Dogru et al., “In vivo confocal microscopy evaluation of meibomian gland dysfunction in atopic-keratoconjunctivitis patients,” in Proceedings of the 64th Congress of Clinical Ophthalmology of Japan, Kobe, Japan, November 2010.
  32. E. Villani, G. Ceresara, S. Beretta, F. Magnani, F. Viola, and R. Ratiglia, “In vivo confocal microscopy of meibomian glands in contact lens wearers,” Investigative Ophthalmology and Visual Science, vol. 52, no. 8, pp. 5215–5219, 2011. View at Publisher · View at Google Scholar · View at Scopus
  33. E. Villani, S. Beretta, M. De Capitani, D. Galimberti, F. Viola, and R. Ratiglia, “In vivo confocal microscopy of meibomian glands in Sjögren's syndrome,” Investigative Ophthalmology and Visual Science, vol. 52, no. 2, pp. 933–939, 2011. View at Publisher · View at Google Scholar · View at Scopus
  34. Y. Ban, Y. Ogawa, O. M. A. Ibrahim et al., “Morphologic evaluation of Meibomian glands in chronic graft-versus-host disease using in vivo laser confocal microscopy,” Molecular Vision, vol. 17, pp. 2533–2543, 2011. View at Google Scholar · View at Scopus
  35. Q. Le, J. Hong, W. Zhu, X. Sun, and J. Xu, “In vivo laser scanning confocal microscopy of vernal keratoconjunctivitis,” Clinical and Experimental Ophthalmology, vol. 39, no. 1, pp. 53–60, 2011. View at Publisher · View at Google Scholar · View at Scopus
  36. O. M. A. Ibrahim, Y. Matsumoto, M. Dogru et al., “In vivo confocal microscopy evaluation of meibomian gland dysfunction in atopic-keratoconjunctivitis patients,” Ophthalmology, vol. 119, no. 10, pp. 1961–1968, 2012. View at Publisher · View at Google Scholar · View at Scopus
  37. E. Villani, M. D. Strologo, F. Pichi et al., “Dry eye in vernal keratoconjunctivitis: a cross-sectional comparative study,” Medicine (Baltimore), vol. 94, no. 42, article e1648, 2015. View at Google Scholar
  38. E. Villani, E. Garoli, V. Canton, F. Pichi, P. Nucci, and R. Ratiglia, “Evaluation of a novel eyelid-warming device in meibomian gland dysfunction unresponsive to traditional warm compress treatment: an in vivo confocal study,” International Ophthalmology, vol. 35, no. 3, pp. 319–323, 2014. View at Publisher · View at Google Scholar · View at Scopus
  39. Q. Wei, Q. Le, J. Hong, J. Xiang, A. Wei, and J. Xu, “In vivo confocal microscopy of meibomian glands and palpebral conjunctiva in vernal keratoconjunctivitis,” Indian Journal of Ophthalmology, vol. 63, no. 4, pp. 327–330, 2015. View at Publisher · View at Google Scholar
  40. L. Agnifili, V. Fasanella, C. Costagliola et al., “In vivo confocal microscopy of meibomian glands in glaucoma,” British Journal of Ophthalmology, vol. 97, no. 3, pp. 343–349, 2013. View at Publisher · View at Google Scholar · View at Scopus
  41. S. Stanek, “Meibomian gland status comparison between active duty personnel and U.S. veterans,” Military Medicine, vol. 165, no. 8, pp. 591–593, 2000. View at Google Scholar · View at Scopus
  42. Y. Matsumoto, Y. Shigeno, E. A. Sato et al., “The evaluation of the treatment response in obstructive meibomian gland disease by in vivo laser confocal microscopy,” Graefe's Archive for Clinical and Experimental Ophthalmology, vol. 247, no. 6, pp. 821–829, 2009. View at Publisher · View at Google Scholar · View at Scopus
  43. Y. Qazi, A. Kheirkhah, C. Blackie et al., “In vivo detection of clinically non-apparent ocular surface inflammation in patients with meibomian gland dysfunction-associated refractory dry eye symptoms: a pilot study,” Eye, vol. 29, no. 8, pp. 1099–1110, 2015. View at Publisher · View at Google Scholar
  44. M. G. Cunniffe, R. Medel-Jiménez, and M. González-Candial, “Topical antiglaucoma treatment with prostaglandin analogues may precipitate meibomian gland disease,” Ophthalmic Plastic and Reconstructive Surgery, vol. 27, no. 5, pp. 128–129, 2011. View at Publisher · View at Google Scholar · View at Scopus
  45. R. Arita, K. Itoh, S. Maeda et al., “Comparison of the long-term effects of various topical antiglaucoma medications on meibomian glands,” Cornea, vol. 31, no. 11, pp. 1229–1234, 2012. View at Publisher · View at Google Scholar · View at Scopus
  46. R. Arita, K. Itoh, S. Maeda et al., “Effects of long-term topical anti-glaucoma medications on meibomian glands,” Graefe's Archive for Clinical and Experimental Ophthalmology, vol. 250, no. 8, pp. 1181–1185, 2012. View at Publisher · View at Google Scholar · View at Scopus
  47. International Dry Eye WorkShop, “The definition and classification of dry eye disease: report of the definition and classification subcommittee of the international dry eye workshop,” The Ocular Surface, vol. 5, pp. 75–92, 2007. View at Google Scholar
  48. A. Alhatem, B. Cavalcanti, and P. Hamrah, “In vivo confocal microscopy in dry eye disease and related conditions,” Seminars in Ophthalmology, vol. 27, no. 5-6, pp. 138–148, 2012. View at Publisher · View at Google Scholar · View at Scopus
  49. A. S. Bacon, S. J. Tuft, D. M. Metz et al., “The origin of keratopathy in chronic allergic eye disease: a histopathological study,” Eye, vol. 7, supplement, pp. 21–25, 1993. View at Google Scholar · View at Scopus
  50. M. Dogru, C. Katakami, N. Nakagawa, K. Tetsumoto, and M. Yamamoto, “Impression cytology in atopic dermatitis,” Ophthalmology, vol. 105, no. 8, pp. 1478–1484, 1998. View at Publisher · View at Google Scholar · View at Scopus
  51. S. J. Tuft, D. M. Kemeny, J. K. G. Dart, and R. J. Buckley, “Clinical features of atopic keratoconjunctivitis,” Ophthalmology, vol. 98, no. 2, pp. 150–158, 1991. View at Publisher · View at Google Scholar · View at Scopus
  52. C. S. Forster and M. Calonge, “Atopic keratoconjunctivitis,” Ophthalmology, vol. 97, no. 8, pp. 992–1000, 1990. View at Publisher · View at Google Scholar · View at Scopus
  53. M. J. Hogan, “Atopic keratoconjunctivitis,” Transactions of the American Ophthalmological Society, vol. 50, pp. 265–281, 1952. View at Google Scholar · View at Scopus
  54. M. Dogru, N. Okada, N. Asano-Kato et al., “Atopic ocular surface disease: implications on tear function and ocular surface mucins,” Cornea, vol. 24, no. 8, supplement, pp. S18–S23, 2005. View at Publisher · View at Google Scholar · View at Scopus
  55. L. Mastropasqua, L. Agnifili, R. Mastropasqua, and V. Fasanella, “Conjunctival modifications induced by medical and surgical therapies in patients with glaucoma,” Current Opinion in Pharmacology, vol. 13, no. 1, pp. 56–64, 2013. View at Publisher · View at Google Scholar · View at Scopus