ChSte7 Is Required for Vegetative Growth and Various Plant Infection Processes in Colletotrichum higginsianum

Figure 4

Mycelial growth and appressorial formation of ChSte7 disruption mutants and complementation strain. (a) The ChSte7 disruption caused abnormal colony. Wild-type strain Ch-1, ChSte7 disruption mutants ΔChSte7-26 and ΔChSte7-48, and complementation strain CΔChSte7-26-20 were grown on PDA plates for 7 days. (b) Increased furcation of mycelial tips and twisted hyphae were observed for the ΔChSte7 mutants. Hyphae of Ch-1, ChSte7 disruption mutants, and complementation strain picked from the edge of the colonies were examined by light microscopy. Scale bar = 10 μm. (c) Appressorial formation was altered in the ChSte7 disruption mutants. Conidial suspensions of Ch-1, ChSte7 disruption mutants, and complementation strain in distilled water were incubated on hydrophobic surface at 25°C for 24 h. Scale bar = 5 μm.

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