In Vitro Protective Effects of Lycium barbarum Berries Cultivated in Umbria (Italy) on Human Hepatocellular Carcinoma Cells

Figure 3

Effect of LBB extract in HepG2 cells. (a) Genotoxic effect, determined by comet assay, after 4 h of treatment with different concentrations of LBBs (200, 600, 1000, 1400, and 1800 μg/mL). MEM is used as negative control, and 1,2,4-benzenetriol (BT), known to induce oxidative damage to DNA, is used as positive control. Results are expressed as mean ± SD of three independent experiments (); (b) acridine orange and DAPI staining for negative control (MEM); (c) same staining for positive control (100 mM of 1,2,4-benzenetriol, BT); (d) same staining for LBB extract (1800 μg/mL). The arrows indicate the death cells stained in blue with DAPI.

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