Research Article
Upregulation of Oxidative Stress Related Genes in a Chronic Kidney Disease Attributed to Specific Geographical Locations of Sri Lanka
Table 1
Primer and probe sequences used for the study.
| | Name | Accession number | Amplicon length (base pairs) | Oligonucleotide sequences 5′–3′ (F: forward, R: reverse, P: probe) | References |
| | GCLC | NM_001498.3 | 149 | F: ACAAGGACGTTCTCAAGTG | Self-designed | | R: AGGATGGTTTGGGTTTGTC | | P: CCTGTCTGGGGAGAAAGTTCTTGAAACTCT |
| | GSTM1 | NM_000561.3 | 121 | F: GCATGATCTGCTACAATCCA | Self-designed | | R: TTGTTTCCTGCAAACCAT | | P: CCTGAAAAGCTAAAGCTCTACTCAGAG |
| | G6PD | NM_000402.4 | 76 | F: TGCCCCCGACCGTCTAC | RTPrimer DB | | R: ATGCGGTTCCAGCCTATCTG | | P: ACTCGTGAATGTTCTTGGTGACGGCC |
| | NLRP3 | NM_004895.4 | 225 | F: CTCCTTTACGCCAGGGTGAG | Self-designed | | R: AGATAGCGGGAATGATGATATGAG |
| | FGF23 | NM_020638.2 | 120 | F: AATCTCAGCACCAGCCACTC | Self-designed | | R: GGAGGCATTGGGATAGGCTC |
| | B2M | NM_004048.2 | 98 | F: TGCCGTGTGAACCATGTGA | Koop et al., 2003 [17] | | R: CCAAATGCGGCATCTTCAA | | P: TGATGCTGCTTACATGTCTCGATCCCACT |
| | GAPDH | NM_001289745.1 | 151 | F: TGACCTCAACTACATGGTTTA | Self-designed | | R: GCCCCACTTGATTTTGGA | | P: CCATGGCACCGTCAAGGCTGA |
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